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PCR – A Powerful Tool for Multiple Applications
Microsynth offers a comprehensive workflow that begins at the sample extraction stage to produce high-quality data. Choosing to outsource your PCR application saves you time and effort, but also provides benefits from technical expertise and facilities dedicated to conducting various PCR-based experiments.
Digital PCR vs. Real-time PCR vs. end-point PCR at a glance:
|Re-arrangement of the sample in thousands of nanoliter end-point PCR reactions allows determination of the absolute concentration of the analyte.
|Measures accumulation of the PCR amplification product as it occurs.
Measures the amount of accumulated PCR product at the end of the PCR cycles.
|Yes, the compartmentalization of the analyte fits a Poisson distribution.
|Yes, data is collected during the exponential growth (log) phase of PCR when the quantity of the PCR product is directly proportional to the initial amount of analyte.
|No, though rough estimation of the amount of the accumulated PCR product gives 'semi-quantitative' results.
Amplification of DNA for:
Digital PCR is the method of choice when:
Real-time PCR is the method of choice when:
End-point PCR is the method of choice when: