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siRNA for Gene Silencing in Screening Applications

siRNA for Gene Silencing
 
 
Microsynth provides high-quality custom siRNA duplexes for RNA interference (RNAi) screening. Our portfolio includes non-modified and modified siRNA, validated control siRNAs, and an integrated siRNA design tool for fast, reliable project setup. All siRNAs are delivered as ready-to-use aqueous solutions for consistent performance.
 

Applications

  • RNAi screening and gene silencing
  • Functional genomics and target validation
  • Pathway analysis
  • High-throughput screening workflows

Why Choose Microsynth siRNA?

  • High-quality siRNA duplexes with MALDI-TOF MS QC
  • Ready-to-use formulation (no resuspension needed)
  • Integrated siRNA design tool with ranking and BLAST support
  • Non-modified and modified siRNA for screening needs
  • Validated control siRNAs (positive and negative)
  • Fast and reliable delivery

siRNA formats

Non-modified siRNA

  • Standard duplexes (17–25 nt) for routine RNAi screening
  • High specificity and reproducibility
  • Delivered as ready-to-use solution

Modified siRNA

  • Improved stability and performance in cells
  • Suitable for advanced screening and challenging targets
  • Common modifications: 2'-OMe, 2'-F, PTO, DNA overhangs, 3'-GalNAc


View specifications (expand)

  • Length: 17–25 nt
  • Concentration: 40 µM siRNA duplex
  • Buffer: 20 mM NaCl, 10 mM Tris-HCl, pH 7.5
  • Purification and scale options available
Unmodified siRNAs

Product Characteristics:

Synthesis scale1
Desalted
HPLC
PAGE
OD2602
[nmol]2
OD2602
[nmol]2
[OD260] 2
[nmol]2
Genomics
on request
7
not available
 not available
0.04 µmol
9
21
4
8
not available
0.2 µmol
16
35
7
16
2
5
1.0 µmol
36
84
16
45
5
11
Higher Scales
on request
 
1 The synthesis scale represents the initial amount of 3' bases (starting material).
2 Yields indicated represent guaranteed yields and apply to a 2x21mer; Calculation: 1 OD = ~2.5 nmol (please note that this calculation is based on sequences with virtually homogenous distribution of the 4 RNA bases)
Modified siRNAs

Product Characteristics:

Synthesis scale
Modified siRNA, 2' OMe, 2' F, PTO, desalted quality, Na+ Form
Modified siRNA, 2' OMe, 2' F, PTO, 3' GalNAc, HPLC purified
OD260
[nmol]
OD260
[nmol]
0.04 µmol
 6.4
20
 0.64 2
0.2 µmol
12.8
 40 1.92
6
1.0 µmol 28.8 90 6.4 20
 
Yields indicated represent guaranteed yields in OD. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 RNA bases.

Control siRNAs

  • Positive controls for validated knockdown
  • Non-targeting negative controls
  • Scrambled controls on request

Controls ensure reliable interpretation of RNAi screening results and are available in multiple scales and formats.

Control siRNAs

 

Name Sense Strand Sequence
(5’ -3’)

Sense Strand
3’ Overhang
(5’ – 3’)

 Anti-Sense Strand
3’ Overhang
(5’ – 3’)
Lamin CUGGACUUCCAGAAGAACA dTdT dTdT
GFP GCAGCACGACUUCUUCAAG dTdT dTdT
Luciferase CGUACGCGGAAUACUUCGA dTdT dTdT
Non-targeting negative control AGGUAGUGUAAUCGCCUUG dTdT dTdT
Scrambled negative control On request
 

siRNA Design Tool

Design your custom siRNA directly in the Microsynth webshop:

  • Automated candidate generation for your target gene
  • Ranking based on Reynolds scoring rules
  • Based on established siRNA design guidelines
  • Integrated NCBI BLAST link for off-target assessment
  • Direct export for ordering

Design your siRNA now and start your screening workflow.

How to Order

Order your siRNA online through the Microsynth webshop:

  • Enter our webshop
  • Select siRNA in the DNA/RNA synthesis section
  • Enter your sequence manually or upload via Excel template

Order your custom siRNA now or contact our team for support with design, controls, or modified siRNA projects.

Frequently Asked Questions

What is siRNA used for?

siRNA is used for gene silencing in RNA interference experiments, for example in functional genomics, target validation, and screening.

Can modified siRNA be used for screening?

Yes, modified siRNA is commonly used in screening applications to improve stability and performance.

Can I design siRNA online?

Yes, our webshop includes an integrated siRNA design tool with ranked suggestions and off-target assessment support.