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Cell Line Authentication (CLA)
Reliable STR-based authentication of cell lines to ensure data integrity and reproducibility. Microsynth provides cell line authentication services for human and animal cell lines, helping you detect misidentification and cross-contamination early.
Why Cell Line Authentication Matters
Cell line misidentification and contamination remain a major issue in biomedical research, affecting an estimated 15–20% of studies.
Authentication ensures:
- Reliable and reproducible experimental results
- Compliance with publication requirements
- Confidence in cell-based assays and downstream analysis
Why Choose Microsynth?
- Reliable STR profiling – standardized DNA fingerprinting for accurate identification
- Broad organism coverage – human, mouse, rat, hamster, dog and more
- Fast turnaround – results typically within one week
- Easy sample handling – shipment at room temperature
- Expert support – guidance on interpretation and troubleshooting
Applications
- Cell line identity verification
- Detection of cross-contamination
- Quality control of cell cultures
- Validation for publication requirements
- Monitoring of long-term cell culture stability
What is Cell Line Authentication?
Cell line authentication is based on short tandem repeat (STR) profiling, a DNA fingerprinting method that compares genetic markers across multiple loci.
The resulting STR profile is matched against reference databases (e.g. Cellosaurus) to confirm identity or detect contamination.
Service Overview
Microsynth performs complete cell line authentication workflows:
- DNA isolation (if required)
- STR amplification and fragment analysis
- Data interpretation and comparison to reference databases
- Delivery of a detailed analysis report
Output & Reporting
You receive a comprehensive analysis report including:
- STR profile of your sample
- Electropherogram data
- Comparison with reference databases
- Identification of potential contamination or mismatches
STR profiling enables detection of contamination down to ~10% levels and provides highly specific identification of cell lines.
Add-on Services
Optional services to extend your analysis:
- Database comparison (Cellosaurus)
- Mycoplasma contamination testing
- Species Identification
Sample Requirements
- 1–5 million cells (pellet)
- Washed and resuspended in ethanol
- Alternatively: ≥50 µl genomic DNA (≥50 ng/µl)
Samples can be shipped at room temperature.
How to Order
- For new customers, please register at our webshop
- Click on Genetic Analysis in the green "Analysis Services" area
- Click on Tubes under Cell Line Authentication and follow the further instructions.
- Microsynth will confirm the receipt of your samples and ship back the analysis results within one week.