ONT Resequencing (Prokaryotic/Eukaryotic)

Explore your genome with long-read precision.
Identify SNVs, indels, and structural variants in any organism—prokaryotic or eukaryotic—with Oxford Nanopore resequencing.

What You Can Achieve

Detect large structural variants, insertions, deletions, or rearrangements
Characterize repetitive or otherwise inaccessible regions
Verify insertions, knockouts, or engineered edits
Suitable for bacteria, fungi, plants, and other eukaryotes

Before You Start

Please clarify:

Which organism(s) do you want to resequence?
What is the approximate genome size?
Are you targeting specific variant types (e.g. SVs, SNVs, insertions)?
Do you already have a reference genome for alignment?
Will you send DNA or tissue/cell samples?

Modular Workflow

Designed to deliver high-confidence variant calls across your entire genome.

Bioinformatics Analysis

A detailed resequencing report including:

Alignment to reference genome
Single nucleotide variants (SNVs)
Small insertions/deletions (InDels)
Structural variants (SVs)
Annotation of detected variants
Summary tables and variant impact categorization

You receive:

Raw and aligned read data (.fastq, .bam)
Annotated variant lists (.vcf, .tsv)
Visual variant reports and summary tables
Quality and alignment metrics

Turnaround Time

Standard resequencing: approx. 1 to 3 weeks

Dependent on genome size, DNA quality, and analysis scope.

Sample Requirements

Option 1: Pre-extracted HMW gDNA

Minimum 2 μg of HMW double-stranded DNA
≥50% of fragments >20 kb
260/280 >1.8 and 260/230 between 2.0–2.2
≥50 μl at ≥40 ng/μl

Option 2: Cell or Tissue Samples (with Extraction)

Suitable for bacteria, fungi, plants, or other eukaryotic tissues
Must be fresh or properly preserved (no RNAlater or ethanol)
We tailor extraction based on species and sample condition
Include known challenges or prior extraction notes