Food Testing Assays for Reliable Molecular Analysis

Validated qPCR and digital PCR assays for food authenticity, safety and regulatory compliance. Microsynth provides highly specific and sensitive molecular assays for species identification, allergen detection, GMO analysis and pathogen testing.

Why Choose Microsynth Food Testing Assays?

Validated assay performance – high sensitivity and specificity for reliable detection
Regulatory-aligned solutions – suitable for food safety and compliance testing
qPCR and dPCR compatibility – flexible use across platforms
Broad assay portfolio – covering multiple targets and applications
Proven performance – supported by scientific publications and validation data

All assays have been developed in collaboration with Dr. Rene Köppel from the Zurich Cantonal Laboratory, an acknowledged expert on the use of cutting-edge analytical systems for food safety testing.

Applications

Food authenticity testing and species identification
Detection of allergens in food products
GMO screening and quantification
Pathogen detection in food and raw materials
Quality control and regulatory compliance

Available Food Testing Assays

Microsynth offers validated assays for both qPCR and digital PCR (dPCR) platforms.
Assays are optimized for sensitivity, specificity and reproducibility depending on the chosen technology.
Select a category below to explore available targets.

Species Identification

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	Re- ferences
AllMeat	1204	Tetraplex	Chicken	Pork	Beef	Turkey		[4,5,6,16]
AllHorse	1206	Tetraplex	Sheep	Pork	Beef	Horse		[7,8,16,17]
AllMilk	1217	Tetraplex	Cow	Goat	Sheep	Water buffalo		[9]
AllPaté	1223	Pentaplex	Chicken	Duck	Goose	Turkey	Pig	[19]
AllWild	1214	Pentaplex	Chamois	Cattle	Roe deer	Red, Sika and Fallow deer	Pig and Wild Boar
AllCheese	1244	Triplex	Sheep	Goat	Cattle
AllVegi	1222	Triplex	Fish	Myostatin (Mammals / Birds)	Plants
AllWasabi	1213	Duplex	Horseradish	Wasabi				[27]
AssignAnimal	1225	This SYB Green real-time PCR kit allows typification of different species in combination with Sanger DNA sequencing. Amplification is based on the conserved cytochrom B region of DNA from mammals, birds and fishes.						[20]

dPCR Assays

Product Name	Article Number	Assay Type	Re- ferences
dNoBA	1243	This digital PCR kit (EvaGreen duplex assay) allows quantitative determination of Non-Basmati rice content in Basmati rice samples.	[28]
dBePo	1218	This digital PCR kit (probe duplex assay) allows quantitative determination of beef and porc in meat samples.	[29]
dMarzipan	1216	This digital PCR kit (probe duplex assay) allows quantitative determination of marzipan and apricot in marzipan products.

Allergen Detection

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	Re- ferences
AllAll A	1200	Tetraplex	Peanut	Celery	Soybean	Hazelnut		[1, 16]
AllAll B	1202	Tetraplex	Cow's milk	Almond	Chicken egg	Sesame		[2, 16]
AllAll G	1231	Tetraplex	Lupine	Almond	Para Nut	Sesame		[22]
AllAll K	1232	Tetraplex	Sesame	Almond	Lupine	Soybean

GMO Testing

1. Screening:

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	Re- ferences
AllGVOScB	1215	Pentaplex	Nos- terminator	35S promoter	Soy (lectin)	Mais (mhmg)	CaMV	[10]
AllGVOScC	1210	Tetraplex	FMV promoter	Bar gene	CP2 / CP4 / EPSPS	Pat gene		[11,16]

2. Soy:

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	Re- ferences
AllSoyA	1212	Pentaplex	Roundup Ready	Mon89788	Soybean Le1 gene	Soy2704	A5547-127	[12,13,16]
AllSoyB	1235	Pentaplex	DP305423-1	DP356043-5	Lectin	CV127-9	Mon87701	[16]
AllSoyC	1239	Pentaplex	Mon87708	Mon87769	Lectin	FG72	Mon87705	[26]

3. Maize

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	Re- ferences
AllMaize C	1227	Pentaplex	Starlink CBH	Mon863r	T25	Maize (mhmg)	Mon810	[10]
AllMaize D	1229	Pentaplex	Bt11	Nk603	Ly038	Maize (mhmg)	Bt176	[10]
AllMaize E	1240	Pentaplex	GA21	DAS-59122-7	Mon89034	Maize (mhmg)	Mon88017	[23, 24, 25]
AllMaize F	1241	Pentaplex	Syngenta3272	MIR604	TC1507	Maize (mhmg)	DP-98140	[23]
AllMaize G	1242	Pentaplex	Mir162 Maize	DAS-40278-9	Mon87460	Maize (mhmg)	EventBt10	[27, 28, 29, 30]

Pathogen Detection

Product Name	Article Number	Assay Type	Marker 1	Marker 2	Marker 3	Marker 4	Marker 5	References
AllBaktA	1219	Pentaplex	E. coli Stx1 Stx2 intimin	Salmonella (incl. Bongori)	Listeria monocytogenes	Campylobacter spp	IPC pUC19	[14]
AllBaktAv2	1236	Pentaplex	E. coli eae/intimin	Salmonella (incl. Bongori)	Listeria monocytogenes	E. coli Stx1 Stx2	IPC pUC19
AllCamp	1221	Tetraplex	Campylobacter jejuni	Campylobacter lari	Campylobacter coli	IPC pUC19		[15]
AllColi	1233	Tetraplex	E. coli spp	E. coli eae/intimin	E. coli Stx1	E. coli Stx2		[18]
AllBaktB	1237	Pentaplex	E. coli	Clostridium perfringens	Staphylococcus aureus	Bacillus cereus	IPC pUC19	[21]

Click on the product name of any of the assays listed in the tables above to download a user guide.

Assay Performance & Validation

Microsynth food testing assays are designed and validated to ensure reliable results across a wide range of food matrices.

High sensitivity for low-level detection
High specificity for accurate target identification
Robust performance across complex sample types
Compatibility with established PCR workflows

Assays are optimized for reproducibility and consistent performance in routine testing environments.

Scientific References

Selected publications demonstrate the performance and reliability of Microsynth food testing assays.

Show references

References and Additional Technical Information

AllAll A

[1] Köppel R. et al. Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Eur. Food. Res. Technol. 230, 367-374 (2009).

AllAll B

[2] Köppel R. et al. Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Eur. Food. Res. Technol. 230, 367-374(2009).

AllMeat

[4] Eugster A. et al. Quantification of beef, pork, chicken and turkey proportions in sausages: use of matrix-adapted standards and comparison of single versus multiplex PCR in an interlaboratory trial. Eur. Food. Res. Technol. 230, 55-61 (2009).

[5] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey. Eur. Food. Res. Technol. 227,1199-1203 (2008).

[6] Accessories: Calculation sheet for normalisation to 100%

AllHorse

[7] Proficiency test done, publication submitted.

[8] Accessories: Calculation sheet for normalisation to 100%

[17] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep. Eur. Food. Res. Technol. 232,151-155 (2011).

AllMilk

[9] Rentsch R. et al. Interlaboratory validation of two multiplex quantitative real-time PCR methods to determine species DNA of cow, sheep and goat as a measure of milk proportions in cheese European Eur. Food. Res. Technol. 236, 217-227 (2013).

AllGVOScB, AllGVOScC

[10] [11] Köppel et al. Two quantitative multiplex real-time PCR systems for the efficient GMO-screening of food products. European Food Research and Technology (DOI); 239 (4): 635-659 (2014).

AllSoyA

[12] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from four transgenic soy Mon89788, A5547-127, Roundup Ready, A2704-12 and lectin. Eur. Food. Res. Technol. 235, 23-28 (2012).

[13] Accessories: Calculation sheet for ΔΔct calculations

AllBakA IPC

[14] Köppel et al (2013) Nonaplex real-time PCR detection of Listeria monocytogenes, Campylobacter, Salmonella and enteropathogene E. coli after universal enrichement in food samples; European Food Research and Technology 237:315–322.

AllCamp

[15] Mayr, A.M. et al. Rapid detection and differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in food, using Multiplex Real-Time PCR. Journal of food protection. 73,241-250 (2010).

Optimal Taq-polymerases for multiplex application like AllAllA,B, AllMeat, AllHorse, AllSoy and AllGVOScC

[16] Köppel R. et al. Proposal for a performance factor to characterize real-time PCR systems, evaluate premixed DNA-Polymerases and assign minimal performance criteria for individual quantitative PCR-runs. In press.

AllColi
[18] Pavliovic et al. Development of a Multiplex Real-Time Polymerase Chain Reaction for simultaneous Detection of enterohemorragic Escherichia coli and enteropathogenic Escherichia coli Strains. Foodborn Pathogens and Disease. 7,801-808 (2010).

AllPaté

[19] Köppel R. et al. Multiplex real-time PCR for the detection and quantification of DNA from duck, goose, chicken, turkey and pork; European Food Research and Technology Volume 236: 1093-1098 (2013).

AssignAnimal

[20] N.V. Morf et al (2013) A multiplex PCR method to identify bushmeat species in wildlife forensics Forensic Science International: Genetics Supplement Series, doi.org/10.1016/j.fsigss.2013.10.104.

AllBaktB

[21] Köppel R, Tolido I, Marti G, Peier M. Detection of DNA from Escherichia coli, Clostridium perfringens, Staphylococcus aureus and Bacillus cereus after simplified enrichment using a novel multiplex real-time PCR system. Eur Food Res Technol 2017;243:521-530.

AllAllG

[22] Waiblinger et al. Ring trial validation of single and multiplex real-time PCR methods for the detection and quantification of the allergenic food ingredients sesame, almond, lupine and Brazil nut Journal of Consumer Protection and Food Safety; 9: 297-310 (2014).

AllMaize

[23] Seong-Hun Lee, Bu-Young Yi, Su-Jeong Kim (2009) Event-specific analytical methods for biotech maize MIR 604 and DAS-59122-7, Journal of the Science of Food and Agriculture, 89, 15, p 2616–2624

[24] European commission, Joint research centre JRC. Institute for health and consumer protection IHCP, Biotechnology and GMO's unit, Ispra (VA) - Italy October 2008 Protocol. Event-specific method for the quantification of Maize Line MON89034 using real-time PCR.

[25] European commission, Joint research centre JRC. Institute for health and consumer protection IHCP, Biotechnology and GMO's unit, Ispra (VA) - Italy March 2010): Protocol MON88017. Event-specific method for the quantification of maize line MON88017 using real-time PCR.

AllSoyC
[26] René Köppel, Thomas Bucher, Anna Frei, Hans-Ulrich Waiblinger (2015) Droplet digital PCR versus multiplex real-time PCR Method for the detection and quantification of DNA from the four transgenic soy traits Mon87769, Mon87708, MON87705, FG72 and lectin, European Food Research and Technology: Volume 241, Issue 4 Page 521-527

AllWasabi
[27] René Köppel, Thomas Bucher (2016) Duplex real‑time PCR for the determination of wasabi (Eutrema wasabi) contents in horseradish (Armoracia rusticana) products applying the ΔΔct‑method, Eur Food Res Technol (2016) 242:1111–1115, DOI 10.1007/s00217-015-2615-7

dNoBa

[28] Thomas Bucher and René Köppel, Duplex digital droplet PCR NoBa for the determination of non-Basmati rice in Basmati rice (Oryza sativa), European Food Research and Technology, published DOI 10.1007/s00217-015-2599-3

dBePo

[29] Köppel et al, Digital duplex versus real-time PCR for the determination of meat proportions from sausages containing pork and beef, European Food Research and Technology
https://doi.org/10.1007/s00217-018-3147-8

How to Order

Select the required assay(s) from the portfolio
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