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Customized qPCR & dPCR Assays

 
 
End-to-end development of PCR assays tailored to your application — from design and synthesis to validation and testing. Microsynth delivers fully functional, application-specific assays ready for research, diagnostics and biologics testing.
 

Why Choose Microsynth Assay Development?

  • End-to-end assay development – design, synthesis and wet-lab validation included
  • Proven assay performance – tested with positive and no-template controls
  • Expertise in qPCR and dPCR – singleplex and multiplex assay design
  • Regulatory-ready validation – assay validation according to ICH guidelines on demand
  • In-house production – primers and probes manufactured under ISO 13485 conditions

Applications

  • Gene expression analysis (qPCR / RT-qPCR)
  • Digital PCR quantification
  • Molecular diagnostics assay development
  • Biologics and gene therapy testing
  • Validation of DNA/RNA-based assays

What is a Customized PCR Assay?

A customized assay is a fully designed and validated PCR-based detection system tailored to a specific target and application.

Microsynth develops assays including primer and probe design, synthesis, performance testing and optimization to ensure reliable and reproducible results.

Assay Development Workflow

  1. Feasibility assessment and project definition
  2. Primer and probe design
  3. Oligonucleotide synthesis
  4. Wet-lab testing (including controls)
  5. Optimization of assay conditions
  6. Validation (optional, e.g. ICH guidelines)
  7. Delivery of a fully functional assay

Assay Features & Capabilities

  • Singleplex and multiplex assay design
  • Use of advanced probe chemistries (e.g. MGB, LNA)
  • Optimization for sensitivity and specificity
  • Compatibility with common qPCR and dPCR platforms
  • Full transparency for MIQE-compliant publication

Validation & Compliance

  • Assay validation according to ICH guidelines (on request)
  • MIQE-compliant assay design and documentation
  • Suitable for research, diagnostics and regulated environments

Integrated Workflow

Customized assays can be combined with additional Microsynth services:

How to Order

Customized assays are developed individually.

  1. Contact Microsynth to define your project
  2. Receive a tailored assay development plan
  3. Start development and validation