Ready-to-Load Run – Sanger Sequencing for Prepared Samples

Submit your ready-to-load samples in 96-well plates and outsource only the sequencing step.
Ideal for laboratories with established workflows for sample preparation, PCR and purification.

Why Choose Ready-to-Load Run?

Designed for experienced labs – keep full control of sample preparation
High-throughput plate sequencing – optimized for 96-well formats
Fast next-day results – reliable turnaround for large sample sets
Cost-efficient outsourcing – no need to maintain your own sequencer
Flexible data processing – adjust analysis parameters (e.g. trimming, base calling)

Applications

High-throughput plasmid sequencing
PCR product sequencing in plate format
Screening workflows
Core facility or automation pipelines

What is Ready-to-Load Run?

Ready-to-Load Run is a Sanger sequencing service designed for laboratories that perform their own sample preparation, PCR cycling and purification.

Samples are submitted in 96-well plates and processed directly on high-throughput capillary sequencers, allowing you to outsource only the sequencing step while maintaining full control over your workflow.

Sample Submission

Samples must be provided in 96-well plates
Ready-to-load DNA (prepared, cycled and purified in your lab)
Compatible with BigDye Terminator chemistry (v1.1 and v3.1)

Output & Data

You receive high-quality Sanger sequencing data including:

Chromatogram files (.ab1)
Base-called sequences
Optional adjusted analysis parameters (e.g. trimming, IUPAC base calling)

Additional Services

96-well plates and sealing materials available on request

How to Order

Procedure to use our Ready-to-Load Run service for sequencing:

Enter our webshop
Click on Plate under "Ready-To-Load Run" and follow the further instructions
Specify in the “comment box” how samples are dissolved (water, formamid) and which Big Dye chemistry is used (1.1 or 3.1).

Important

Send samples after dye removal in water or formamid.