Back to top

miCORE Resequencing

Whole Genome Sequencing
 
 
High-resolution mutation detection across entire genomes. miCORE resequencing provides accurate insights into SNPs, InDels, and structural variants with Illumina sequencing. Whether for CRISPR off-target validation, strain verification, or comparative genomics, our resequencing service delivers the data you need to study genetic variation at scale

What You Can Achieve

  • Detect inherited or acquired mutations, from SNPs to large structural variations
  • Compare genomes across strains, isolates, or individuals
  • Verify on-target edits and identify off-target mutations from CRISPR/Cas9 experiments
  • Confirm genetic modifications in breeding and cell line studies
  • Study genome-wide differences linked to disease or phenotype

Before You Start

To set up your resequencing project effectively, consider:

  • Scientific objective – mutation detection, genome comparison, CRISPR validation
  • Reference genome quality – completeness and annotation status
  • Sequencing depth – coverage needed for SNV and InDel calling
  • Read length – optimal for resolution of complex variants
  • Sample purity – avoid substantial DNA contamination

Our experts support you from experimental design to bioinformatics analysis.

Modular Workflow

Choose a full-service package or individual modules. A typical resequencing project includes:

Workflow Resequencing

Bioinformatics Analysis

Our resequencing analysis module provides a wide range of deliverables:

  • Data quality control
  • Alignment and genome coverage analysis
  • SNV and small InDel detection (VCF files)
  • Structural variation and copy number analysis
  • Annotation of variants with amino acid-level consequences (for annotated references)
  • Optional: background filtering, consensus sequence assembly, genomic epidemiology (prokaryotes)

Results are delivered in standard formats (.vcf, .bam, .bed, .html, .pdf) with summary reports for easy interpretation.

Example Results

[below] from left to right

  • Genome coverage histogram
  • Single nucleotide variations: small insertions and deletions and their annotation
  • Putative structural variations
  • Copy number variation: in relation to a reference sample

All results are delivered in publication-ready figures and interactive formats, providing both high-level overviews and nucleotide-level detail.

Turnaround Time

  • Sequencing only: 20 working days
  • Data analysis (bioinformatics): +3 working days (small genomes) or +6 working days (large genomes)
  • Express service available upon request

Sample Requirements

  • DNA buffer: 10 mM Tris-HCl (pH 7.5–8.5)
  • Avoid: EDTA >1 mM
  • DNA quantification: Fluorometric methods (e.g., PicoGreen®, Qubit®)

Contact us for project-specific requirements regarding DNA amount and quality.