Amplicon Deep Sequencing

Pinpoint mutations. Detect rare variants with confidence.
Whether for CRISPR verification, metabarcoding, or mutation profiling—amplicon deep sequencing delivers precise and reliable results, even for low-frequency events.

What You Can Achieve

Identify single nucleotide variations (SNVs) and small insertions and deletions (InDels)
Detect rare variants with high sensitivity
Analyze targeted regions with high throughput
Investigate specific regions of interest within a complex DNA background
Parallelize Sanger sequencing for short DNA regions

Before You Start

To optimize your setup from design to analysis, consider the following:

What is the length and location of your target region?
How complex is your sample? What sequencing depth is required?
What is the optimal amount and quality of DNA for your analysis?

Modular Workflow

Outsource the entire workflow—or select specific modules. Our process is designed for flexibility. Typical workflow steps include:

Bioinformatics Analysis

We offer customized analysis options to meet your specific research needs. For diversity detection, we provide:

Data quality control
Read clustering/dereplication
Detection of SNVs and InDels relative to a reference sequence, including impact analysis at the protein level
Comparative analysis and principal component analysis (PCA)

Turnaround Time

15–20 working days for sequencing
+3 working days for full analysis
Express delivery available on request

Sample Requirements

Buffer recommendation: 10 mM Tris-HCl (pH 7.5–8.5)
Important: Avoid any buffers containing EDTA >1mM
DNA quantification: Use fluorometric methods (e.g., PicoGreen®, Qubit®)

Sample amounts required for Illumina sequencing:

Type of Library	Amount (µg)	Concentration (ng/µl)
1st or 2nd step Nextera PCR product	>0.2	>5

Notes

We recommend reaching out to our team for guidance on primer design and multiplexing strategies.
Custom setups and additional downstream analyses are available upon request.