ChIP Sequencing

Investigate transcriptional regulation and active chromatin regions
ChIP sequencing allows for the genome-wide identification of transcription factor binding sites, histone modifications, and chromatin states. It helps you understand how gene regulation is altered across different biological conditions.

What You Can Achieve

Detect genome-wide transcription factor binding sites
Identify the impact of histone modifications on chromatin structure
Explore regulatory changes under various experimental conditions

Before You Start

To ensure optimal experimental design, consider the following questions:

Is a suitable reference genome available for alignment?
Are you targeting narrow peaks (e.g., transcription factors) or broad peaks (e.g., histone modifications)?
What sequencing depth is appropriate for your biological question?
Have you defined replicates, controls (e.g., input DNA), and experimental conditions?

Modular Workflow

Outsource the entire workflow—or select specific modules. Our process is designed for flexibility. Typical workflow steps include:

Bioinformatic Analysis

Our standard bioinformatics package includes:

Peak detection: Identification of enriched binding regions across the genome
Motif analysis: Discovery of known and novel DNA-binding motifs
Pathway analysis: Insight into potentially affected cellular pathways

Results are provided in both raw and analyzed formats—ready for downstream interpretation or publication.

Turnaround Time

25 working days from sample receipt for library preparation and sequencing
+10 working days for data analysis
Express service available upon request

Sample Requirements

Buffer recommendation: 10 mM Tris-HCl (pH 7.5–8.5)
Important: Avoid any buffers containing EDTA >1mM
DNA quantification: Use fluorometric methods (e.g., PicoGreen®, Qubit®)

Sample Amounts for Illumina Sequencing:

Type of Library	Amount (µg)	Concentration (ng/µl)
ChIP-Seq libraries	>0.05	>5