ONT De Novo Sequencing (Eukaryotes)

What You Can Achieve

• High-quality genome reconstructions for eukaryotic organisms
• Long reads >20 kb enable superior resolution of repetitive or complex regions
• Functional annotation using homology-based tools
• Tailored protocols for difficult samples (e.g. rich in secondary metabolites)

Before You Start

Please clarify:

• Which eukaryotic organism(s) do you plan to sequence?
• Do you require DNA extraction or will you send purified HMW DNA?
• Are there known challenges (e.g. secondary metabolites, tough cell walls)?
• hat is the approximate genome size (in Mb)?

Modular Workflow

Customized for robust de novo genome assembly of eukaryotes.

Bioinformatics Analysis

Your genome is reconstructed and functionally annotated with homology-driven tools:

• High-quality de novo genome assembly
• Gene prediction and annotation based on related reference organisms
• Functional classification of coding regions
• Standard output formats for GenBank, GFF, and genome browsers

You receive:

• Polished genome assembly (.fasta)
• Gene prediction & annotation files (.gb, .gff, .tsv)
• Functional classification tables
• Assembly metrics and summary reports

Turnaround Time

• <30 Mb genome: approx. 7 business days
• <100 Mb genome: approx. 3–4 weeks

Turnaround depends on DNA quality and genome complexity. Challenging samples may require longer processing time.

Sample Requirements

Option 1: Pre-extracted HMW gDNA

• Minimum 2 μg of high-purity, HMW double-stranded DNA
• >50% of DNA >20 kb in size
• Purity: 260/280 > 1.8 and 260/230 between 2.0–2.2
• Volume: ≥50 μl at ≥40 ng/μl
• DNA QC not included—please verify quality before shipping

Option 2: Cell or Tissue Samples (with Extraction)

• Accepted: fresh, flash-frozen, or preserved fungal or plant tissues
• Must be free from RNAlater or ethanol
• Custom DNA isolation protocols can be integrated for difficult samples
• Include organism info and known sample challenges in submission form