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Off Target Analysis

Despite the reported precision of CRISPR/Cas9 editing, growing evidence shows that off-target edits do occur. With complex engineering schemes that employ multiple guide RNAs, even chromosomal translocation have been observed. Microsynth can assist by monitoring potential off-target sites by amplicon deep sequencing.

Deep Sequencing Analysis

InDel frequencies caused by CRISPR/Cas9 or any other genome-editing technology at suspected off-target sites can be quantified using an amplicon deep sequencing strategy. Microsynth offers to run the full analytical procedure from DNA isolation to bioinformatics analysis.