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Frequently Asked Questions - Next Generation Sequencing

Samples and library preparation

Do you conduct a quality control before library preparation?

Yes we conduct a thorough entry quality control of your samples. Nevertheless, ensure to do your own quality control before you ship the samples in order to avoid delays or other pitfalls in processing.

How long do you store my DNA/RNA samples and custom primers?
  • Samples are stored for 3 months
  • Custom NGS primer are stored for 2 years
When should I use NextGen and when should I use Sanger technology for amplicon sequencing?

This is rather difficult to answer by general means, best you contact us to discuss your project in detail.

How should I arrange the samples in a 96-well plate to make sample processing as easy as possible?

Please fill all wells column-wise.
For projects comprising more than 4 x 96-well plates please get in touch with us to discuss an efficient plate design.

Sequencing

How do sequencing depth, read length and replicates impact my project?

To put it simple:

  • Sequencing depth means sensitivity
  • Read length means specificity
  • Replicates mean confidence

Bioinformatics

How long do you store my data?

Data is stored for 3 months.

Do you analyse already existent NGS-data?

Yes we do, please see our services and contact our bioinformatics specialists.

Why do I need a reference genome?

Beside resequencing many applications such as transcriptome sequencing rely on a high quality reference genome as basis for the deeper analysis. The full reference sequence ensures where to count, while the full reference annotation ensures what to count. Additional annotations of a reference genome allows additional deeper analysis such as pathway analysis, alternative transcript detection,…

What if there is no reference genome or only a draft assembly of the genome?

There are always work arounds – please see our service „Reference Transcriptome Sequencing“ and contact us for details.