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Frequently Asked Questions - Next Generation Sequencing
Samples and library preparation
Yes we conduct a thorough entry quality control of your samples. Nevertheless, ensure to do your own quality control before you ship the samples in order to avoid delays or other pitfalls in processing.
- Samples are stored for 3 months
- Custom NGS primer are stored for 2 years
This is rather difficult to answer by general means, best you contact us to discuss your project in detail.
Please fill all wells column-wise.
For projects comprising more than 4 x 96-well plates please get in touch with us to discuss an efficient plate design.
To put it simple:
- Sequencing depth means sensitivity
- Read length means specificity
- Replicates mean confidence
Data is stored for 3 months.
Beside resequencing many applications such as transcriptome sequencing rely on a high quality reference genome as basis for the deeper analysis. The full reference sequence ensures where to count, while the full reference annotation ensures what to count. Additional annotations of a reference genome allows additional deeper analysis such as pathway analysis, alternative transcript detection,…