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Full PlasmidSeq

Microsynth’s state-of-the-art full plasmid sequencing service is based on long-read sequencing technology. It will reconstitute the sequence of your plasmid using a sophisticated bioinformatic pipeline developed by Microsynth that recreates the closed-circle plasmid sequence including annotation from the raw sequencing reads. The service is available for samples in tubes and 96-well plates.

Features and Benefits

  • Results delivered within 1 to 3 days following sample receipt
  • Results are delivered Monday to Friday
  • Use our drop box in your vicinity for free shipping of samples.
  • You can choose between prepaid and non-prepaid service.
  • Hypothesis-free sequencing of the complete plasmid.
  • No primers needed.

  • Economical way of sequencing your complete plasmid.
Complete Information
  • You will receive the annotated circular contig of your plasmid.
  • Detailed supporting information to evaluate sequence quality and identify problematic regions.
  • High accuracy.



How can I ship the samples?

Use our drop box system, or send your samples by mail.

What amount of DNA and concentrations do you need?

>15 µl at 20 ng/µl

Technical Questions

How can I judge the quality of the received sequence?

We supply additional information that allow you to identify problematic regions and evaluate the overall sequencing quality:

  • In the *.variable.tsv file that opens with Excel, all residues of the contig are listed that show a rate >10% of mismatches, deletions and/or insertions. These values are based on all the reads that were used to create the contig. You may sort the list according to % mismatches, % deletions, or % insertions to identify the regions within your plasmid that might be problematic or ambiguous. The coverage, as well as base quality of the reads at the respective position are also indicated.
  • In the *.cleaned.html file of the addendum you can see a statistics of the reads that were used for producing the assembly, which comprises number of reads, length of reads and quality score of reads.
The results indicate mutations in critical regions, which potentially could delay the projects planned with the plasmid. How can I be sure that the mutations are real?

Please consult the additional information to judge the sequence quality at the position. For critical applications we recommend you to double check the sequence with an orthogonal sequencing method such as Sanger or Illumina.

I have received a plasmid including map that I have sequenced with your Full PlasmidSeq pipeline. The results communicated by you are different from my previous map. Can I call your support to discuss the discrepancies?

Please understand that we cannot provide scientific support at this level. Our support is limited to technical questions regarding sequencing quality, sample quality or technical abnormalities in the sequencing.

How can I open/view the data in certain files (eg. tsv, gbk)?

If you are working on Windows you may want to switch on viewing of file name extensions (In File Explorer under View, in the Show/hide group, select the File name extensions check box). For instance tab separated value files (.tsv) may be opened in any text editor or by drag and drop in an open but empty Excel table. Fasta (.fasta) and genbank (.gbk) files may be opened in a text editor or by specialized software.

How to Order

Procedure to order labels for our Full PlasmidSeq:
  • Enter our webshop
  • Click on Full PlasmidSeq in the "Order Labels" area and follow the further instructions
  • You can choose between prepaid and non-prepaid labels
Procedure to use our Full PlasmidSeq for sequencing.

  • Enter our webshop
  • Click on Fill Order Form under Full PlasmidSeq (prepaid or non-prepaid) and follow the further instructions
  • Enter our webshop
  • Click on Fill Order Form under Full PlasmidSeq and follow the further instructions
See also Microsynth's user guide on the right side for more detailed information.