Sample Requirements for Sanger Sequencing

Ensure optimal sequencing results by preparing your samples according to the guidelines below.
For detailed instructions, please refer to the corresponding user guides.

DNA Amount (Volume & Concentration)

The required DNA amount depends on both concentration and sample volume.

Template Type	Concentration	Volume	Total DNA
Plasmid DNA	40–100 ng/µl	12 µl : Tubes 10 µl : Plates	400–1500 ng
PCR product (purified and unpurified)*	General Rule: 1.5 ng per 100 bp	12 µl : Tubes 10 µl : Plates	Depends on length

*Upfront PCR purification is highly recommended but can easily be outsourced to Microsynth.

E. coli Samples

50 µl LB medium OR visible colony
Ecoli NightSeq: 1 colony (~1 mm) or 5 µl culture

Primer Requirements

Primer Type	Concentration	Volume
Premixed	4 pmol/µl = 4 µM	12 µl DNA template solution + 3 µl 20µM sequencing primer solution
Separate primer submission	10 pmol/µl = 10 µM	20 µl + 3 µl per additional reaction

Sample Types

Purified plasmid DNA
PCR products
E. coli (colony or liquid culture for Ecoli NightSeq)

Sample Handling & Shipping

Use tubes or 96-well plates
Ensure proper labeling
Seal samples securely

Samples can be shipped at room temperature.

Quick Checklist

DNA amount within recommended range
Correct primer concentration
Samples clearly labeled
Tubes or plates properly sealed

Detailed Instructions

For detailed sample preparation guidelines, please refer to the corresponding user guides: