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Nanopore (ONT) Yeast Genome Sequencing

 
 
Obtain complete yeast genomes with high-quality annotations — fast and reliable.
Ideal for de novo assembly from clonal yeast populations using Oxford Nanopore’s long-read technology.

What You Can Achieve

  • Generate high-quality, annotated genome assemblies
  • Receive rich assembly metrics and visualization tools
  • Sequence entire chromosomes without PCR-induced artifacts

Hypothesis-free sequencing of complete, native yeast genomes

Before You Start

To prepare your project efficiently, please clarify:

  • Will you send purified genomic DNA or yeast cell pellets?
  • Do you prefer standard or hybrid assembly (with short-read polishing)?
  • If sending DNA: Have you verified concentration and purity of high-molecular-weight gDNA?

Workflow

A modular, optimized pipeline for yeast whole-genome sequencing:

Oxford Nanopore Yeast Genome Sequencing

Bioinformatics Analysis

Your yeast genome is assembled and annotated :

  • Polished de novo genome assembly
  • Gene prediction and annotation
  • Assembly completeness assessment using BUSCO
  • Raw read and contig quality metrics
  • Interactive HTML summary report

You receive:

  • Raw reads (.fastq.gz)
  • Polished genome (.fasta)
  • Gene annotations (.gff and .gbk)
  • Summary report (.html)
  • Contig metrics, BUSCO scores, and visual stats (.png/.tsv/.txt)

If you provide a reference genome you will also receive variant calling and coverage analysis.

Turnaround Time

  • 10 business days with pre-extracted genomic DNA
  • 15 business days with yeast cell DNA extraction

Sample Requirements

Option 1: Pre-Extracted Genomic DNA

Type Genome Size Min. Conc. Min. Volume
Genomic DNA < 20 Mb 50 ng/μl 20 μl

DNA Quality Requirements:

  • Double-stranded, high-molecular-weight (HMW) DNA (>50% >15 kb)
  • ≥1 μg total input (≥1.5 μg if Illumina polishing is requested)
  • Purity: 260/280 > 1.8; 260/230 between 2.0–2.2
  • Preferred buffer: 10 mM Tris-HCl (pH 8) with 1–2 mM EDTA

Please confirm DNA quality before shipping.


Option 2: Yeast Cell Pellets (for DNA Extraction)

  • Resuspend only in NAP buffer (no RNAlater or other preservatives)
  • BSL-1 and lysozyme-accessible BSL-2 strains accepted
  • Send pellets from exponential or early stationary phase cultures
  • Ensure sufficient biomass to avoid failed extraction

Note: We do not culture cells — DNA is extracted directly from your provided material.