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Ready-to-Load Sequencing

Ready-to-Load Sequencing
 
 
You prepare the libraries — we sequence them.
Our Ready-to-Load (RTL) Sequencing services are designed for experienced users who perform their own library preparation. Choose the platform that best suits your project:

  • NovaSeq – High-throughput, cost-efficient sequencing in data packages
  • MiSeq – Dedicated flow cells, flexible read modes, and long reads for targeted applications

Platform Overview

Platform Ideal For Read Modes Index Type Max Multiplexing TAT
NovaSeq High-throughput sequencing 2×150 bp / 2×250 bp Unique Dual Indexes (UDI) Kit-dependent ~10 business days
MiSeq Amplicons, long reads, small sets 1×50 to 2×300 bp Combinatorial Dual Indexes (CDI; Nextera only) Up to 384 ~5 business days

NovaSeq – Ready-to-Load

What You Can Achieve

  • Generate high-quality sequencing data from your own library prep
  • Choose between 2×150 bp or 2×250 bp paired-end read modes
  • Sequence libraries using your preferred indexing system (from our supported list)
  • Minimize turnaround time and maximize flexibility

Sample Requirements

  • DNA concentration: ≥10 nM
  • Volume: ≥20 μl per library pool
  • Library QC required (size distribution, fluorometric quantification)
  • Barcode sheet with UDI index set information

Supported Index Sets for NovaSeq (UDI Only)

Only the following unique dual index (UDI) systems are supported:

  • Roche KAPA UDI Adapter Kit / Primer Mixes (1–384)
  • NEBNext Multiplex Oligos (Set 1–5, 96 UDI Pairs)
  • Takara Bio UDI Kits (1–384)
  • Twist Universal Adapter System (Plate A–D)
  • Revvity NEXTFLEX UDI Barcodes (384 Set)
  • Illumina TruSeq & Tagmentation Indexes (Set A–D)
  • IDT for Illumina – TruSeq RNA/DNA UD v2
  • Agilent SureSelect XT HS2 Index Primer Pairs (1–384)

MiSeq – Ready-to-Load

What You Can Achieve

  • Sequence amplicon libraries with Nextera adapters
  • Choose read lengths from 1×50 up to 2×300 bp
  • Enjoy dedicated MiSeq flow cells (no pooling with others)
  • Multiplex up to 384 samples per run

Sample Requirements

  • Libraries must be purified amplicons with Nextera adapters
  • DNA concentration: ≥10 nM
  • Volume: ≥20 μl per sample
  • Provide QC data and barcode information

Supported Index Sets for MiSeq (CDI Only)

Only the following Combinatorial Dual Index (CDI) system is accepted:

  • Illumina Nextera DNA Indexes (CDI format only)

Other formats, including UDI systems, are not accepted with this MiSeq service.

Ordering & Submission Instructions

  1. Visit: shop.microsynth.com
  2. Navigate to: Illumina Sequencing → Ready-to-Load Sequencing
  3. Select NovaSeq or MiSeq, depending on your library type
  4. Fill out the order form including index set used and sample metadata
  5. Print the order confirmation and enclose with your shipment