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Long PCRSeq – Nanopore Amplicon Sequencing

 
 
High-quality sequencing of PCR amplicons up to 50 kb with Oxford Nanopore long-read technology

Microsynth’s Long PCRSeq service is designed for researchers who need complete consensus sequences of PCR products rather than plasmids. Using Oxford Nanopore long-read sequencing, Long PCRSeq provides accurate results for clonal linear DNA fragments ranging from 600 bp to 50 kb. This amplicon sequencing service builds on the proven technology of our Full PlasmidSeq, delivering the same speed, convenience, and data quality – optimized for PCR amplicons.

 

Highlights

  • Fast turnaround – Consensus data within 1–2 working days after sample receipt (Monday–Friday).
  • Flexible sample formats – Submit amplicons in tubes or 96-well plates; free drop-box shipping available.
  • No primers required – Hypothesis-free sequencing of the complete PCR product.
  • Cost-effective – Economical solution for sequencing linear DNA fragments without cloning.
  • Comprehensive output – Receive polished consensus sequences plus detailed quality assessment.

Bioinformatics

With Long PCRSeq you will receive:

  • Consensus sequences in multiple formats (.fasta, .fastq, .ab1).
  • Annotation provided as GenBank (.gbk) and interactive HTML reports.
  • Uncertainty analysis including detailed quality metrics for sequencing and assembly.

Turnaround Time

  • Results within 1–2 working days after sample receipt
  • Results delivered Monday to Friday

Use our Microsynth drop boxes for free and convenient shipment.

How to Order

Ordering Long PCRSeq is identical to Full PlasmidSeq.

Procedure to order labels for our Full PlasmidSeq/Long PCRSeq:

  1. Enter our webshop.
  2. Select Tubes or Plates under Full PlasmidSeq / Long PCRSeq.
  3. Choose between prepaid and non-prepaid labels.
  4. Generate labels, prepare samples, and ship.

Procedure to use our Full PlasmidSeq/Long PCRSeq for sequencing.

  1. Enter our webshop
  2. Click on Tubes or Plates under "Full PlasmidSeq / Long PCRSeq" and follow the further instructions

For more detailed instructions, please consult Microsynth’s User Guide.