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Nucleobase Modifications (for RNA)
| Nucleobase Modifications | Position | ||
| 5’ | 3’ | int | |
| RNA-Inosine (INO) | X | X | |
| Deoxyuridine | X | ||
| Mixed Bases | X | ||
RNA-Inosine (INO)
Historically, the first universal base employed was 2’-deoxyInosine (dI). DeoxyInosine is a naturally occurring base that, while not truly universal, is less destabilizing than mismatches involving the four standard bases. Hydrogen bond interactions between dI and dA, dG, dC and dT are weak and unequal, with the result that some base-pairing bias does exist with dI:dC > dI:dA > dI:dG > dI:dT. When present in a DNA template, deoxyInosine preferentially directs incorporation of dC in the growing nascent strand by DNA polymerase.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| 3' |
X
|
X
|
O |
X
|
X | ||
| Int |
X
|
X
|
O |
X
|
X | ||
X = available/on stock
O = on request
Deoxyuridine
DNA analogue of RNA-U.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int |
X
|
X
|
O |
X
|
X | ||
X = available/on stock
O = on request
Mixed Bases
Modification used for site-specific randomization.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | O | O | O | O | O | ||
X = available/on stock
O = on request