Sample Requirements for DNA Profiling & Cell Culture QC

Use the overview below to prepare samples for Fragment Length Analysis, Cell Line Authentication and Mycoplasma Testing.
For detailed preparation steps, please refer to the corresponding user guides or contact Microsynth.

Service Overview

Service	Sample Type	Required Format	Key Requirement
Fragment Length Analysis	Labeled PCR products	96-well plate only	≥10 µl per well
Cell Line Authentication	Cell pellet or isolated DNA	Tube	1–5 million cells or ≥50 µl gDNA
Mycoplasma Testing	Cell culture supernatant	Tube	150 µl heat-inactivated supernatant

Fragment Length Analysis

Samples for Fragment Length Analysis must be submitted in 96-well plates. Individual tubes cannot be processed.

Requirements:

≥10 µl diluted, labeled PCR product per well
Plate must be labelled with a Microsynth FLA barcode plate label
Ready-to-load samples must already contain size standard / Hi-Di formamide mix
Empty wells must be filled with distilled water
Plates must be sealed properly for safe shipment

For detailed preparation instructions, please refer to the Fragment Length Analysis User Guide.

Technical Details

Show me filter sets

Filter Sets

Dye Set Name	Matrix Type	Blue	Green	Yellow	Red	Orange
Powerplex_4C	4 dye	FAM	JOE	TAMRA	CXR
Powerplex_4C-RCT	4 dye	FAM	JOE	TAMRA	CXR
DS-30_D	4 dye	6-FAM	HEX	NED	ROX
DS-30_D-RCT	4 dye	6-FAM	HEX	NED	ROX
DS-33_G5	5 dye	6-FAM	VIC	NED	PET	LIZ
DS-33_G5-RCT	5 dye	6-FAM	VIC	NED	PET	LIZ
Mic-G5	5 dye	FAM	ATTO532	ATTO550	ATTO565	Dyomics630
Mic-G5-RCT	5 dye	FAM	ATTO532	ATTO550	ATTO565	Dyomics630

RCT: This dye set reduces signal intensity and potential crosstalk of capillaries. Higher concentration peaks can be used without going offscale.

Filter set and size standard must be specified during ordering and are the customer’s responsibility.

Show me size standards

The table below lists the size standards compatible with each dye set.

Size Standard	Size Standard Range	Fluorophor	ABI Channel	Compatible Filter Set
GeneScan 120 LIZ	15 - 120 nt	LIZ	Orange	DS-33_G5 Mic-G5
GeneScan 350 ROX	35–350 nt	ROX / CXR	Red	Powerplex_4C DS-30_D
GeneScan 400 HD ROX	50–400 nt	ROX / CXR	Red	Powerplex_4C DS-30_D
GeneScan 500 LIZ	35–500 nt	LIZ	Orange	DS-33_G5 Mic-G5
GeneScan 500 ROX	35–500 nt	ROX / CXR	Red	Powerplex_4C DS-30_D
GeneScan 600 LIZ	20–600 nt	LIZ	Orange	DS-33_G5 Mic-G5
ILS 600 CXR	60–600 nt	ROX / CXR	Red	Powerplex_4C DS-30_D
MapMarker 1000 ROX	50–1000 nt	ROX / CXR	Red	Powerplex_4C DS-30_D
GeneScan 1200 LIZ	20–1200 nt	LIZ	Orange	DS-33_G5 Mic-G5

RCT option: Dye sets using the RCT signal setting use the same size standards as the corresponding standard dye sets.

Cell Line Authentication

For Cell Line Authentication, submit either a cell pellet or isolated genomic DNA.

Accepted sample types:

Cell pellet: 1–5 million cells, washed twice in PBS or another appropriate buffer, resuspended in 0.5 ml of 70–90% ethanol
Isolated DNA: ≥50 µl genomic DNA at 50 ng/µl in Tris or low-EDTA buffer

Mycoplasma Testing

For Mycoplasma Testing, collect cell culture supernatant from cultures grown for at least 72 hours without medium change. Cell cultures should be approximately 80–90% confluent, but not overgrown.

Sample preparation overview:

Collect cell-free culture supernatant
Centrifuge to remove cells and debris
Transfer 150 µl supernatant into a 1.5 ml or 2 ml flip-cap tube
Heat-inactivate at 95°C for 10 minutes
Label the tube with a Microsynth Mycoplasma barcode label

For detailed preparation instructions, please refer to the Mycoplasma User Guide.

Quick Checklist

Before sending your samples:

Correct sample format selected
Required volume provided
Barcode label attached
Plate or tube properly sealed
Service-specific user guide checked, if applicable

Detailed Instructions

For complete preparation instructions, please refer to:

For Cell Line Authentication, the sample requirements above are sufficient in most cases. Contact Microsynth if your sample type differs from the listed options.