NGS Adapters & Indexing Oligos for Reliable Sequencing

Applications

• Library preparation for next-generation sequencing
• Target enrichment and capture workflows
• Indexing and sample multiplexing
• Amplicon sequencing
• Custom NGS workflows

NGS Adapters and Indexing Oligos

We design and manufacture custom oligonucleotides for demanding sequencing applications:

• Custom NGS adapters for library preparation
• Indexing primers and barcoding oligos
• Compatible with Illumina and other sequencing platforms
• Optimized designs to minimize cross-contamination

Why Choose Microsynth NGS Oligos?

• High sequence fidelity for accurate sequencing results
• Stringent quality control for reproducibility
• Optimized workflows to minimize cross-contamination
• Flexible customization for complex designs
• Fast and reliable turnaround times

Synthesis Scales

Microsynth has established and validated a specific process for the preparation of NGS oligonucleotides, based on a rigorous HPLC purification procedure in which the elimination of cross-contamination is a top priority.

Available Modifications and Formats

We support a wide range of NGS-specific requirements, including:

• 5'-phosphorylation for ligation workflows
• Custom adapter designs
• Indexing and barcoding sequences
• Fluorophores and labels
• Additional custom modifications upon request

Quality Control and Manufacturing Standards

All NGS oligonucleotides are manufactured under strict quality standards and undergo comprehensive quality control:

• Analytical HPLC
• Mass spectrometry (MALDI-TOF or ESI-MS)

This ensures high purity, correct sequence identity, and reproducibility—critical for reliable sequencing data.

How to Order

Option 1: When ordering NGS primers, please proceed as follows:

• Enter our webshop, click on DNA in the blue “DNA/RNA Synthesis” domain
• Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering)
• Follow the further instructions

Option 2: When ordering primers for Illumina amplicon sequencing, please proceed as follows:

• Use the dedicated “Order Form_Illumina_AmpliconDeepSeq” (can be downloaded in the right column); in this context see also our application note “Amplicon Deep Sequencing”)
• Specify now the locus-specific sequences for your first-step PCR primers and then select your desired indexed forward and reverse primers
• Enter our webshop, click on DNA in the blue “DNA/RNA Synthesis” domain and then on Upload Entry
• Follow the further instructions