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Spacers (for DNA)

 
Spacers Position
  5’ 3’ int
C3 Spacer   X X
Spacer 18     X
Spacer C12     X
dSpacer (abasic side) O O X
 

C3 Spacer

 
The C3 Spacer phosphoramidite can be incorporated internally or at the 3'-end of the oligo. Multiple C3 spacers can be added at the 3'-end of an oligo to introduce a long hydrophilic spacer arm for the attachment of fluorophores or other pendent groups.
 
 
Position
Synthesis Scale [µmol]
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
3'  
X
X
O  
X
X
Int  
X
X
O  
X
X
X = available/on stock
O = on request
 

Spacer 18

Spacer 18
 
Spacer 18 is an 18-atom hexa-ethyleneglycol spacer. It is the longest spacer arm that can be added as a single modification.
 
 
Position
Synthesis Scale [µmol]
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
Int  
X
X
O  
X
X
X = available/on stock
O = on request
 

Spacer C12

Spacer C12
 
Spacer C12 is a 12-carbon spacer that is used to incorporate a long spacer arm into an oligonucleotide. Spacer C12 is frequently used for solid-phase immobilization of DNA probes or aptamers for microarray applications, but can be used for any oligonucleotide-based application requiring a long spacer arm.
 
 
Position
Synthesis Scale [µmol]
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
Int  
X
X
O  
X
X
X = available/on stock
O = on request
 

dSpacer (1’,2’-Dideoxyribose)

dSpacer
 
The 1’,2’-Dideoxyribose modification is used to introduce a stable abasic site within an oligonucleotide.
 
 
Position
Synthesis Scale [µmol]
Purification
 
0.04
0.2
1.0
15
Des
HPLC
PAGE
Int  
X
X
O  
X
X
X = available/on stock
O = on request