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Quenchers (for DNA)
|
Type of Modification
|
Position
|
Quenching Range
|
||
|
5’
|
3’
|
Int.
|
(nm)
|
|
|
X
|
480-580 / 560-670 | |||
| Dabcyl |
X
|
400-550 | ||
| IQ-500 |
X
|
400-550 | ||
| MGB-Q500 | X |
|
400-550 | |
| MGB-Q530 | X |
|
450-620 | |
Black Hole Quencher-1
BHQ-1 is best suited for fluorophores that emit in the wavelength range from FAM to TAMRA.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
|
5’
|
O | O |
O
|
|
|||
|
3’
|
|
X
|
X
|
O
|
|
X
|
|
X = available/on stock
O = on request
Black Hole Quencher-2
BHQ-2 is best suited for fluorophores in the Cy3 to Cy5 wavelength range.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
|
5’
|
O | O |
O
|
|
|||
|
3’
|
|
X
|
X
|
O
|
|
X
|
|
X = available/on stock
O = on request
Dabcyl
Dabcyl is a relatively weak dark quencher and is best suited for use in molecular beacon probes where the hairpin places the quencher and fluorescent dye in close proximity.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
|
3’
|
|
X
|
X
|
O
|
|
X
|
X
|
X = available/on stock
O = on request
Internal Quencher IQ-500
IQ-500 (internal quencher 500) is a fluorescence quencher unit that is incorporated internally into an oligonucleotide. Thus it can be positioned at optimal FRET distance relative to a fluorophore. IQ-500 is mainly used as internal quencher of double quenched qPCR probes.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int |
|
X
|
X
|
O
|
|
X
|
|
X = available/on stock
O = on request
MGB-Q500
MGB-Q500 is a fluorescence quencher unit attached to a minor groove binding (MGB) residue. This 3'-modification allows the design of shorter, yet highly specific qPCR probes. It is best suited in combination with 5' FAM. MGB-Q500 is a cost-effective alternative for MGB-Q530.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| 3' |
|
X
|
X
|
O
|
|
X | |
X = available/on stock
O = on request
MGB-Q530
MGB-Q530 is a very effective fluorescence quencher unit attached to a minor groove binding (MGB) residue. This 3'-modification allows the design of shorter, yet highly specific qPCR probes with very low noise level. It is best suited in combination with 5' FAM/HEX/JOE/Yakima Yellow.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| 3' |
|
X
|
X
|
O
|
|
X | |
X = available/on stock
O = on request