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Miscellaneous (for DNA)
| Miscellaneous | Position | ||
| 5’ | 3’ | int | |
| 3’ Deoxy-A, 3’ Deoxy-C, 3’ Deoxy-G, 3’ Deoxy-T | O | X | X |
| Inverted dA, dT, dG, dC | X | ||
3’ Deoxy-A, 3’ Deoxy-C, 3’ Deoxy-G, 3’ Deoxy-T
3’-Deoxy-A, C, G and T nucleotides are functional equivalents to 2'3' Dideoxy-A, C, G and T nucleotides. 3'-Deoxy nucleotides are incorporated into oligonucleotides at the 3’ end for use in structure/activity investigations and as chain terminators in PCR reactions.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| 5' | O | O | O |
|
O | O | |
| 3' |
X
|
X
|
O |
X
|
X | ||
| Int |
O
|
O
|
O |
O
|
O | ||
X = available/on stock
O = on request
Inverted dA, dT, dG, dC
Capping at the 5'-end for protection against exonuclease digestion.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | X | X | O | X | X | ||
X = available/on stock
O = on request