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Exigences par rapport aux échantillons

 
 
Veuillez prendre un peu de temps pour examiner nos exigences en matière d'échantillons. En suivant ces recommandations, vous améliorez la qualité de vos résultats et vous nous donnez la possibilité de vous aider davantage.
 
 

Concentration

 
Template Concentration
Plasmid 40-100 ng/µl
PCR (purified and unpurified products)*
 
Length 100 - 400 bp
Length 401 - 900 bp
Length 901 - 2000 bp
General Rule: 1.5 ng/µl per 100 bp
 
2 - 6 ng/µl
4 - 17 ng/µl
12 - 70 ng/µl
E.coli
50 µl LB Medium OR
visible colony on agar plate
E.coli (Ecoli NightSeq®)
add 1 colony (diameter 1 mm) OR
add 5 µl turbid liquid culture
Plasmid (Full PlasmidSeq)
20 ng/µl
Genomic DNA (BacterialSeq)
50 ng/µl
*La purification PCR en amont est fortement recommandée mais peut facilement être sous-traitée à Microsynth.
 
 
Primer
Concentration
Premixed
4 pmol/µl = 4 µM
As separate tube (enclosed) 10 pmol/µl = 10 µM

Volumes

 

Service Volume Additional Information
Economy Run (Tube)
12 µl
Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
Economy Run (Plate)
10 µl/well in 96-well plate
Increase the volume by 5 µl /well for each additional reaction
Premium Run
At least 20 µl
Increase the volume by 5 µl for each additional reaction
Primer Walking Service
At least 20 µl

Single Stranded

0.5 µg of plasmid-DNA for every kb of your construct

 

Double stranded

1 µg of plasmid-DNA for every kb of your construct

Full PlasmidSeq
At least 15 µl

Suitable plasmid sizes range from 2.5 kb to 25 kb. While plasmids ranging from 25 kB to 50 kb have not been tested yet, you may try them at your own risk.

BacterialSeq
At least 20 µl

High Quality DNA is needed for this services. That means high-molecular weight DNA with no fragments <5 kb.

Primer Volume
As separate tube (enclosed) 20 µl + 3 µl per additional reaction

 

Stockage

 

Service
Storage at Microsynth
Economy Run (Tube)
4 days
Economy Run (Plate)
1 month
Premium Run
3 months
Primer Walking Service
3 months
Full PlasmidSeq (Tube)
4 days
Full PlasmidSeq (Plate)
1 month
BactSeq (Tube)
1 month
Primer (stored)
4 months
Primer (custom primer list)
10 months