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nanoCORE Amplicon Metagenomics

Amplicon Metagenomics
 
 
Explore microbial communities with Microsynth's nanoCORE Amplicon Metagenomics service, powered by Oxford Nanopore sequencing. Offering full-length, marker-based sequencing, our service delivers unmatched taxonomic resolution down to the species level. Whether you're assessing environmental samples, tracking changes in agricultural soils, or investigating microbial diversity in medical contexts, our precise and efficient service supports your research with the highest accuracy.

 

Features and Benefits


Fast
  • Results delivered within 3 weeks of sample receipt
 
Convenient
  • Complete service bundle: From sample isolation to advanced library preparation, sequencing, and comprehensive bioinformatic analysis
  • Use our drop box in your vicinity for free shipping of samples.
  • Get started with your analysis using just one sample!
 

Cost-Effective
  • An economical way to explore your microbial community.
  • Select the stages you want to begin and complete for your samples, and we’ll provide highly competitive fixed rates tailored to your specific needs.
 
Enhanced Flexibility
  • No size restriction of the marker locus
  • Sequencing of length polymorphisms
  • Increased taxonomic resolution
 

Workflow

 
The workflow for the nanoCORE Amplicon Metagenomics service is shown in the graphic below. Please note that our modular process allows you different entry options.
 

For further reading and a detailed technical description, please download our flyer and user guides (see related downloads).

Results

Standard Bioinformatic Analysis

With Microsynth's standard bioinformatics analysis module for nanoCORE Amplicon Metagenomics, you'll receive comprehensive insights:

  • Comprehensive report (in interactive .html format)
    This detailed report guides you through the data, allowing sorting and filtering of key results interactively.
  • Amplicon Sequence Variants (ASVs)
    ASVs, also known as zero-radius operational taxonomic units (zOTUs), in .fasta format.
  • ASV Abundance, Taxonomy, and Prediction Confidence (in .tsv format)
    Insights into ASV abundance, taxonomy, and prediction confidence.
  • Intra- and Intersample Diversity Analysis and Rarefaction Curves (in .tsv and .pdf format, see Figure 1 and 2)
    Explore intrasample diversity and estimate metagenomic coverage using rarefaction curves.
  • Metagenome Visualization via Krona Charts (in interactive .html format, see Figure 3)
    Easily interpret and explore your metagenomic data through interactive Krona Charts.
  • Principal Component Analysis (in .tsv and .pdf format)
    Visualize and interpret complex relationships through principal component analysis.
  • Differential ASV Analysis (in .tsv and .html format)
    Identify and explore differential ASVs with detailed analysis results available in both tabular and HTML formats.
  • Visualization of Statistical Analysis
    Access visually informative representations of statistical analyses.

These results provide a comprehensive understanding of your experiment, from an overview down to the nucleotide level, empowering you to make informed decisions and draw meaningful conclusions. 

Figure 1: Alpha Diversity

Figure 1: Alpha diversity measures for the analyzed community including observed richness, Simpson indices and the Shannon diversity indices representing the estimated richness and evenness.

Figure2: Rarefaction Curves

Figure 2: Rarefaction curves depicting alpha diversity across samples from various origins, evaluated through observed species richness, Shannon diversity, and Simpson diversity indices.

Figure 3 : Krona Chart

Figure 3: Picture of an interactive Krona chart illustrating a mock community.

Turnaround Time

  • Delivery of data within 10 working days upon sample receipt (includes library preparation, sequencing and data analysis/bioinformatics)
  • Additional 5 days for DNA isolation