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Ecoli NightSeq

 
 
Innovative new Sanger sequencing service in 96-well plates for plasmids. Drop your E. coli colonies into a Microsynth drop box, receive your result the next day from 3 pm on and accelerate your research by one day.


*only valid for drop boxes in Switzerland and Germany that are connected to our same-day courier system.
 

Features and Benefits

 
Additionally to the features and benefits you know from the Economy Run, the Ecoli NightSeq offers following benefits:
 
Fast
  • Overnight service, delivery of results from 3 pm on the next day
  • Results are delivered Monday to Friday
 
Convenient
  • Use our drop box in your vicinity for free shipping of samples
  • You can choose between prepaid and non-prepaid service
 
Robust
  • Ideal for difficult plasmids. Better and more robust results because whole process is standardized and carried out in-house.
 
Cost-Effective
  • Accelerate your research by one working day
  • Save handling time in the lab
  • Significantly reduce your expenditures on plasmid DNA purfication kits by only isolating plasmids from clones showing the desired sequence
 
Versatile
  • Not limited to E. coli. Expanding list of possible organisms.
 

Additional Services

 
 
The Ecoli NightSeq service is also available in the single tube format.
 

How to Order

 
 
Procedure to order labels for our Ecoli NightSeq service:
 
  • Enter our webshop
  • Click on Ecoli NightSeq in the "Barcode Labels for Plate Services" area and follow the further instructions
  • You can choose between prepaid and non-prepaid labels
 
Procedure to use our Ecoli NightSeq for sequencing:
 
  • Enter our webshop
  • Click on Fill Order Form under Ecoli Nightseq (prepaid or non-prepaid) and follow the further instructions
 
See also Microsynth's user guide on the right side for more detailed information.

FAQ

Technical Questions

Do I have to add antibiotics to the liquid?

No, an antibiotic is not necessary.

Do I really have to pick from a colony that is at least 1 mm in diameter?

The colony may be a bit smaller, but it must be clearly visible.

I want to inoculate the Ecoli NightSeq® tubes and streak out on an agar plate with the same toothpick. What should I do first: inoculate the ENS tube or streak out my agar plate?

First the ENS tube.

Does ENS work for low copy plasmids as well?

Yes.

I do not work with agar plates, but with liquid culture. Can I also inoculate the ENS Tubes with liquid culture?

Yes, please add 5 µl.

For how long does Microsynth store my Ecoli NightSeq® samples?

1 month.

Does the Ecoli NightSeq® also work for BACs or other organisms?

Yes, the Ecoli NightSeq® also works for other gram-negative bacteria such as Agrobacterium tumefaciens and Pseudomonas. There is a chance that BACs or other organisms such as yeast will also produce acceptable sequencing results. If you are working with other organisms than E. coli, we strongly recommend performing pilot tests beforehand.

 

Stability

How long can I use Ecoli NightSeq® tubes and plates? How shall I store them?

Storage time: 4 years for Ecoli NightSeq® tubes, 2 years for Ecoli NightSeq® plates. Storage conditions: 4 °C for Ecoli NightSeq® tubes, - 20 °C for Ecoli NightSeq® plates. Expiry date and storage conditions are indicated on the packaging bag.

Several reactions from the same clone or additional reactions later

I would like to pick a clone and do a forward and a reverse reaction on it. Is that possible?
Yes, but you must provide the sample in two wells. The procedure is as follows: Inoculate one well and mix and divide the fluids of both wells.
What you should not do: Inoculate one well and then inoculate the other well with the same toothpick, because then you have an unequal cell density.
I would like to have a second reaction with a different primer after I received the first reaction. Is that possible?
No, it is not possible to sequence single wells from a plate with a different primer.

Logistics

I do not have a Microsynth drop box in my vicinity. May I also mail it, even if it stays over the weekend?

Yes. We have tested a correspondingly long storage at room temperature and it doesn’t harm the sample.

Do I receive the results on Saturday, when I drop the samples on Friday into the drop box?

No, only on Monday before 4 pm.

How should I send / label the sequencing primer?
The same way as with our other types of services.
Concentration 10 µM, at least 20 µl volume and write the name of the primer on the tube.

Testimonials

 
 
“Thanks to the new Ecoli NightSeq service from Microsynth, we are now able to sequence in high-throughput format and save up to two days compared to the previous service. Time really is money for us since we can significantly shorten critical project timelines. The easy sample preparation, the high-quality sequences, the long read-lengths as well as the competent, fast and uncomplicated customer support were convincing us, too.”
Andreas Lehmann, Molecular Partners AG

 

“We’ve been using the Ecoli NightSeq service proposed by Microsynth for 6 months now and have only seen advantages to the system. It allows you to save one day in your cloning process by sending directly your colonies for sequencing. The most important aspect is that you do not need to extract the DNA saving bench time and money. This reduces in effect the general price of the read. We’ve been surprised by the quality of the reads which you cannot distinguish from extracted DNA reads. Overall, it’s a plus!”

Thomas Lunardi, EPFL SV ISREC UPLIN

 

“I have used the Ecoli NightSeq service primarily to test whether a site directed mutagenesis has worked, as well as to check cloned CrisprCas9 plasmids. For such applications this service is very convenient, as they need to be confirmed with sequencing anyways and hence Ecoli NightSeq allowed me to save unnecessary minipreps.”

Barbara Hauert, University of Bern

 

“The main benefits of Ecoli NightSeq are saving one day as well as saving time and consumable costs for the mini-prep of 3-5 picked colonies from each clone. Another benefit is that Microsynth Seqlab provides the option for standard primers, which makes life easier for many constructs. In terms of quality, whenever poor sequence reads were found, Microsynth Seqlab has repeated the sequencing with no extra cost. Taken together, I’m extremely satisfied with the new Ecoli NightSeq service, an additional high standard sequencing service from Microsynth Seqlab.”

Dr. Shubhendu Palei, TU Dortmund

 

Product Video

 
 
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